Antibiofilm activity of Cyanobacteria spirulina as an irrigation solution against Enterococcus faecalis

Sodium hypochlorite (NaOCl) is currently the golden standard for root canal irrigation. NaOCl at a concentration of 5.25% to 6% can eliminate E. faecalis, but this increase risk toxic effects. Cyanobacteria spirulina known produce several secondary metabolites that have antimicrobial activity against gram-positive and gram-negative bacteria. The aim study was determine antibiofilm power biofilms Enterococcus faecalis concentrations 60 mg/ml, 70 80 90 mg/ml. This research true experiment with post-test only group design. object divided into 6 groups. Group 1 negative control group, 2 positive 5% NaOCl, 3, 4, 5, were treatment groups given solution respectively. Optical density (OD) bacteria bound by staining analyzed ELISA auto reader wave length 595 nm (OD nm). results LSD test showed significance between K+ P1, P2, P3 (p< 0.005), P4 (p= 0.129), P1 P2 (p=0.449), (p=0.178). Significance p<0.0005 significant difference data using one-way ANOVA followed double comparison Least Different (LSD) Post Hoc method. mg/ml had greatest inhibitory effect on biofilm formation faecalis.